D3252
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D3252
Sigma-Aldrich
o-Dianisidine dihydrochloride
Purified for use with peroxidase and peroxidase-coupled reactions.
| Synonym: | 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B |
| CAS Number: | 20325-40-0 |
| Linear Formula: | C14H16N2O2 · 2HCl |
| Molecular Weight: | 317.21 |
| Beilstein Registry Number: | 3917996 |
| EC Number: | 243-737-5 |
| MDL number: | MFCD00012488 |
| PubChem Substance ID: | 24893736 |
Description
| Specificity | o-Dianisidine (3,3'-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl. |
| Warning | Probably carcinogenic. |
| Physical form | crystalline |
Properties
| solubility | H2O: soluble |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type P2 (EN 143) respirator cartridges |
| Hazard Codes | T |
| Risk Statements | 45-22 |
| Safety Statements | 53-45 |
| WGK Germany | 3 |
| RTECS | DD1050000 |
References
| Beilstein | Beil. 13,IV,2834 |
